The smart cycler software ensures ease of use for the sixteen independently controlled icore modules found on the smart cycler processing block. During pcr cycling, optical signals from two sequence specific fluorescent probes within the reaction are monitored in real time. Maxima probe qpcr master mix, rox solution provided. For the biopharma industry investment, business development and competitive intelligence professionals who require information to support financing, partnering and licensing activities, bciq provides accurate information and context to support profitable and strategic decision making. Cepheid s is staffed with experienced technical support specialists and scientists. Pdf the ability of the portable cepheid smartcycler realtime pcr. Post a free request for proposal for a cepheid smartcycler ii thermal cycler automated realtime pcr system and get quotes from our gold service dealers.
Cepheid smart gbs test for group b streptococcus, run on. Unlike other intelligence solutions, bciq exclusively supports the unique needs of the biopharma industry and. Cepheid smartcycler automated realtime pcr system thermal cycler 16 site. I agree that cepheid shall have no obligation to pay me or otherwise compensate me in any way for receiving and evaluating the submission, and that any decision by cepheid to partner, purchase or license the submission or any intellectual property associated. Rapid and sensitive detection of mycobacterium dna using. Cepheid accessory for smartcycler pcr systems, cepheid. Evaluation of the portable cepheid smartcycler realtime.
Find and purchase cepheid smartcycler products at invitrogen life science technologies. Laboratory considerations for use of cepheid xpert mtbrif. Cepheid genotyping is becoming more important in many clinical settings for disease diagnosis and optimized treatment choices. Method 1609 describes a quantitative polymerase chain reaction qpcr procedure for the detection of dna from enterococci bacteria in ambient water matrices based on the amplification and detection of a specific region of the large subunit ribosomal ribonucleic. Cepheid smart cycler 9000330 automated realtime pcr. Cepheid s genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. Cepheid launches ce mark certification smart ebvtm in vitro diagnostic test for smartcyclerr platform read this article along with other careers information, tips and advice on biospace. However, there are several obstacles to clinical viability, including time required for testing, cost, and interpretation of. Description of the cepheid smartcycler starter system, desktop. Cepheid smart cycler ii td realtime pcr system govdeals.
Csu surplus property is selling a cepheid smart cycler ii td realtime pcr system previously used in a university setting. The smartcycler systems optics allow fluorescence to be measured in four channels at the same time. Concentration, extraction, and direction of norovirus and. Upon completion of run, transfer purified nucleic acid to nucleasefree tubes.
Each reaction site can be programmed and run independently. Cepheid smartcycler ii thermal cycler automated realtime. Realtime pcr detection of shigatoxin producing escherichia coli stec, serotype o157 and non o157 in fresh produce and food with nono157 isolation and identification revision. For full access to this pdf, sign in to an existing account, or. Information on this page may be partial or incomplete and may contain mistakes or inaccurate information. Find out why cepheids solutions are a top choice for fast, accurate, and easy diagnostic testing. Evaluation of the smartcycler ii system for realtime. Each of the standard 16 icore modules expandable to 96 requires no warmup time, no routine maintenance, and no normalization dye. The cepheid smart cycler dx system is an integrated nucleic acid amplification and detection instrument system based on the proprietary microprocessorcontrolled icore module.
The smart cycler settings consisted of a primary curve analysis with a manual threshold setting of 10, background subtraction turned on, boxcar average set to five cycles, background minimum cycle set to 5, and background maximum cycle set to 45. Method 1611 describes a quantitative polymerase chain reaction qpcr procedure for the detection of dna from enterococci bacteria in ambient water matrices based on the amplification and detection of a specific region of the large subunit ribosomal rna gene lsrrna, 23s rrna from these organisms. Pdf evaluation of the portable cepheid smartcycler real. Manufacturer specifications smartcycler ii td, cepheid. Smartcycler ii the smartcycler is a realtime pcr instrument with 16, 34, 48, 64, 80 or 96 independent reaction sites. Cepheid launches ce mark certification smart ebvtm in. Evaluation of the smartcycler ii system for realtime detection of.
Cepheid smartcycler sc1600 starter system, desktop medex. You are also free to make these changing later on while. For further questions please contact cepheid in the foyer. Cepheid smart cycler 9000330 automated realtime pcr system. Michael t taylor, william mcmillan, rapid and sensitive detection of mycobacterium dna using cepheid smartcycler. Cepheid smartcycler ii td pcr manufacturer specifications. Cepheid smart cycler ii rtpcr model sc2 with 16 well block, including computer with software. Our cepheid trained staff is available for onsite customer training for all cepheid.
The smartcycler system at the heart of the smartcycler system is the icore module a reliable, solidstate optical system with no moving parts. The companys systems enable rapid, sophisticated molecular testing for organisms and geneticbased diseases. If you require additional information or technical assistance, please feel free to. The cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical reaction module. Pdf evaluation of the portable cepheid smartcycler realtime. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smartcycler system software program the software on the computer provided with the smart. Designing science equipment for the international space station and beyond. Cepheid warrants that the smartcycler processing block the instrument i is free from. The staff is available 12 hours a day, via toll free number, by email, or through the cepheid web site. Preparation of a master mix, which includes all reaction components except template dna, helps to avoid pipetting errors and is an essential step in realtime pcr. Thermal cyclers cepheids smart cycler thermal cycler system monitors the accumulation of pcr products by performing both amplification and immediate.
The reagents for the amplification reaction on the cepheid smartcycler were provided in the euroclone duplica real time dual easy ct assay. Type ii cepheids are variable stars which pulsate with periods typically between 1 and 50 days. Additionally, by uploading or otherwise transmitting material to any area of the web site you. For the smart cycler instrument, data acquisition and analysis were done by using cepheid smart cycler software version 1. Find out why cepheid s solutions are a top choice for fast, accurate, and easy diagnostic testing. Cepheid enabling access to molecular diagnostic testing.
Cepheids smart cycler instrument facilitates the speed and accuracy of the idistrep b test. The detection of microbial dna in clinical samples by nucleic acid amplification methods is often limited by the low concentration of organisms present in the s. For the smart cycler, data were analyzed by using the cepheid smart cycler software version 1. Cepheid launches ce mark certification smart cmv in vitro. If you require additional information or technical assistance, please feel free to email our technical support group at. We designed, optimized, and extensively tested several sensitive and specific realtime pcr assays for rapid detection of both smallpox and panorthopox virus dnas. United states department of agriculture agricultural. Details about cepheid smart cycler 9000330 automated realtime pcr system.
Cepheid smartcycler ii thermal cycler automated realtime pcr system 10268. Smartcycler sc1600 starter system includes a processing block, software, operators manual, accessory kit, and desktop computer with cdrw drive and flatpanel monitor. Smartcycler icore module independently programmable unit. The test can provide a highly accurate result in as little as 30 minutes, during the critical period when detection of gbs may potentially reduce the number of infections and deaths to. Amplification and detection on the cepheid smart cycler. Please refer to the smartcycler operator manual, appendix b, and the. Toxoplasmosis in a woodchuck marmota monax and two. In the thresh setting column, enter manual threshold for each channel influenza a, influenza. Cepheid launches ce mark certification smart cmv in vitro diagnostic test for smartcycler pcr platform. Thermal cycler spectral calibration instructions why is spectral calibration necessary.
Txrd and cy5 channels smart cycler operator manual. This is a 510k application for a new qualitative realtime polymerase chain reaction pcr assay used with the cepheid smartcycler ii instrument for the in vitro qualitative. If further optimization is needed, determine the optimal concentration within the range of 0. Cepheid smart cycler sample processing module extraction and purification of rna or dna in microgravity glovebag to reduce risk for cross contamination with nonsample rna or dna. Cepheid inc is an american molecular diagnostics company, now part of danaher, that develops, manufactures and markets fully integrated systems for testing in the clinical market, and for application in its original nonclinical market. Evaluation of cepheid smartcycler and euroclone duplicareal time. Detection of food pathogens using the smart cycler ii gene. Genomic dna up to 500 ng and plasmid dna up to 10 ng can be used in qpcr with maxima probe qpcr master mix, no rox. Cepheid smart gbs test for group b streptococcus, run on the smartcyclerr system, receives 510k fda clearance read this article along with other careers information, tips and advice on biospace. Smallpox and panorthopox virus detection by realtime 3.
Medwow disclaims any obligation, responsibility or liability which concerns or relates to any information. Dnasernase free water applied biosystems molecular grade. Computer is windows 2000 with cepheid smart cycler version 2. Our it staff was able to power on the smart cycler and included laptop. Data acquisition and analysis were done with cepheid smart cycler software version 2. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the realtime pcr assay currently in use in the laboratory. Cepheid smartcycler system software license agreement. Objective to compare the smartcycler ii system cepheid, sunnyvale, calif results with those of standard cell culture, to compare the smartcycler ii system results with those of a dedicated polymerase chain reaction facility, and to establish the smartcycler ii system as a polymerase chain reaction method for detecting viral and chlamydial dna from ocular specimens. Acros organics, fisher scientific, inverting the tube 30 to 40. Objectiveto compare the smartcycler ii system cepheid, sunnyvale, calif. Cepheid smartcycler system software license agreement license grant.
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